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Addgene inc david ron
David Ron, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/david+ron/ATF4+5%3A+5%E2%80%99ATF4%2EGFP+(Plasmid+%2321852)/pm41896223-87-9-11
Average 93 stars, based on 9 article reviews
david ron - by Bioz Stars, 2026-09
93/100 stars

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Bimolecular Fluorescence Complementation Assay:

Article Title: CDNF rescues human iPSCs-derived dopamine neurons through direct binding to unfolded protein response sensors PERK and IRE1α
Article Snippet: .. To assemble the pEZY BiFC pre-NV(CV)-PERK and pEZY BiFC pre-NV(CV)-PERK KDD constructs, we amplified the PERK/PERK cDNA from plasmids gifted from David Ron (Addgene #21814 and #21815). ..

Construct:

Article Title: CDNF rescues human iPSCs-derived dopamine neurons through direct binding to unfolded protein response sensors PERK and IRE1α
Article Snippet: .. To assemble the pEZY BiFC pre-NV(CV)-PERK and pEZY BiFC pre-NV(CV)-PERK KDD constructs, we amplified the PERK/PERK cDNA from plasmids gifted from David Ron (Addgene #21814 and #21815). ..

Article Title: Enhanced depletion of MLL-fusion proteins in acute leukemia: potential for improved therapeutic outcomes
Article Snippet: .. Lentivirus vector constructs 3 The wild-type (eIF2αWT) and mutated eIF2 (eIF2αS51A) overexpression constructs were a gift from David Ron (eIF2a 1 and 2, Addgene plasmids #21807 and #21808), and were cloned into the pCSGW-PIG lentivirus expression construct.2 .. Total RNA was isolated using the RNAeasy Mini Kit (Qiagen) and RNA concentration was measured using a NanoDrop ND-1000 (Labtech International).

Amplification:

Article Title: CDNF rescues human iPSCs-derived dopamine neurons through direct binding to unfolded protein response sensors PERK and IRE1α
Article Snippet: .. To assemble the pEZY BiFC pre-NV(CV)-PERK and pEZY BiFC pre-NV(CV)-PERK KDD constructs, we amplified the PERK/PERK cDNA from plasmids gifted from David Ron (Addgene #21814 and #21815). ..

Plasmid Preparation:

Article Title: TurboID-Based IRE1 Interactome Reveals Participants of the Endoplasmic Reticulum-Associated Protein Degradation Machinery in the Human Mast Cell Leukemia Cell Line HMC-1.2
Article Snippet: QIAquick gel extraction (#M0530) and DNA purification kits (#51306/#12662) were from Qiagen (Hilden, Germany). .. The following plasmid were used in this study: Flag_HsIRE1a_pBabePuro was a gift from David Ron (Addgene plasmid #54337; http://n2t.net/addgene:54337 (accessed on 22 February 2019); RRID:Addgene_54337) and has been described in Volmer et al. [ ]. ..

Article Title: Targeting glioblastoma mitochondrial metabolism with S-Gboxin induces cytotoxicity under conditions of the tumor microenvironment.
Article Snippet: .. The ATF4 5: 5’ATF4.GFP plasmid was a gift from David Ron (Addgene plasmid # 21852; http://n2t.net/addgene:21852; RRID: Addgene_21852) (24) and was used to generate ATF4-reporter cells by repeated sorting (Suppl. ..

Article Title: CYP1B1 Mediates Cigarette Smoke–Induced Lipid Accumulation in Alveolar Type 2 Cells
Article Snippet: Commercial siRNA of CYP1B1 (siCYP1B1, Catalog # AM16708, Assay ID 145515 or s64677) and siRNA control (siCon) were purchased from Thermo Fisher Scientific. .. The CYP1B1 plasmid was designed and subcloned into pBABE (Addgene, MA, USA). pBABE was a gift from David Ron (Addgene plasmid # 21836). .. Lipofectamine 2000 (Thermo Fisher Scientific) was used for transfection according to the manufacturer's protocol.

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans.
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2AEGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or PhoenixAmpho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Article Title: Induction of proteasomal activity in mammalian cells by lifespan-extending tRNA synthetase inhibitors.
Article Snippet: Tavaborole was purchased from Cayman Chemical (23101). .. Fluorescence and luciferase assays in MEFs ATF4 translation experiments utilized a fluorescent translational reporter for ATF4:ATF4 5:5’ATF4.GFP was a gift from David Ron (Addgene plasmid # 21852; http:// n2t. net/ addge ne: 21852; RRID:Addgene_21852) [36]. ..

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2A-EGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or Phoenix-Ampho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Article Title: Enhanced depletion of MLL-fusion proteins in acute leukemia: potential for improved therapeutic outcomes
Article Snippet: .. Lentivirus vector constructs 3 The wild-type (eIF2αWT) and mutated eIF2 (eIF2αS51A) overexpression constructs were a gift from David Ron (eIF2a 1 and 2, Addgene plasmids #21807 and #21808), and were cloned into the pCSGW-PIG lentivirus expression construct.2 .. Total RNA was isolated using the RNAeasy Mini Kit (Qiagen) and RNA concentration was measured using a NanoDrop ND-1000 (Labtech International).

Sequencing:

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans.
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2AEGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or PhoenixAmpho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2A-EGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or Phoenix-Ampho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Synthesized:

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans.
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2AEGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or PhoenixAmpho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2A-EGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or Phoenix-Ampho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Clone Assay:

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans.
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2AEGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or PhoenixAmpho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Article Title: CMTM6 suppresses cell-surface expression of death receptor FAS in mice but not in humans
Article Snippet: .. The coding sequence of PD-L1-Alfa, in frame with T2A-EGFP, was synthesized and cloned into the pBabe Puro vector, provided by David Ron (Addgene plasmid #21836). .. Phoenix-Eco cells (for mouse cell transduction) or Phoenix-Ampho cells (for human cell transduction) were transfected with the retroviral vectors using Lipofectamine 2000 (Thermo Fisher Scientific) to produce retroviral particles.

Article Title: Enhanced depletion of MLL-fusion proteins in acute leukemia: potential for improved therapeutic outcomes
Article Snippet: .. Lentivirus vector constructs 3 The wild-type (eIF2αWT) and mutated eIF2 (eIF2αS51A) overexpression constructs were a gift from David Ron (eIF2a 1 and 2, Addgene plasmids #21807 and #21808), and were cloned into the pCSGW-PIG lentivirus expression construct.2 .. Total RNA was isolated using the RNAeasy Mini Kit (Qiagen) and RNA concentration was measured using a NanoDrop ND-1000 (Labtech International).

Fluorescence:

Article Title: Induction of proteasomal activity in mammalian cells by lifespan-extending tRNA synthetase inhibitors.
Article Snippet: Tavaborole was purchased from Cayman Chemical (23101). .. Fluorescence and luciferase assays in MEFs ATF4 translation experiments utilized a fluorescent translational reporter for ATF4:ATF4 5:5’ATF4.GFP was a gift from David Ron (Addgene plasmid # 21852; http:// n2t. net/ addge ne: 21852; RRID:Addgene_21852) [36]. ..

Luciferase:

Article Title: Induction of proteasomal activity in mammalian cells by lifespan-extending tRNA synthetase inhibitors.
Article Snippet: Tavaborole was purchased from Cayman Chemical (23101). .. Fluorescence and luciferase assays in MEFs ATF4 translation experiments utilized a fluorescent translational reporter for ATF4:ATF4 5:5’ATF4.GFP was a gift from David Ron (Addgene plasmid # 21852; http:// n2t. net/ addge ne: 21852; RRID:Addgene_21852) [36]. ..

Over Expression:

Article Title: Enhanced depletion of MLL-fusion proteins in acute leukemia: potential for improved therapeutic outcomes
Article Snippet: .. Lentivirus vector constructs 3 The wild-type (eIF2αWT) and mutated eIF2 (eIF2αS51A) overexpression constructs were a gift from David Ron (eIF2a 1 and 2, Addgene plasmids #21807 and #21808), and were cloned into the pCSGW-PIG lentivirus expression construct.2 .. Total RNA was isolated using the RNAeasy Mini Kit (Qiagen) and RNA concentration was measured using a NanoDrop ND-1000 (Labtech International).

Expressing:

Article Title: Enhanced depletion of MLL-fusion proteins in acute leukemia: potential for improved therapeutic outcomes
Article Snippet: .. Lentivirus vector constructs 3 The wild-type (eIF2αWT) and mutated eIF2 (eIF2αS51A) overexpression constructs were a gift from David Ron (eIF2a 1 and 2, Addgene plasmids #21807 and #21808), and were cloned into the pCSGW-PIG lentivirus expression construct.2 .. Total RNA was isolated using the RNAeasy Mini Kit (Qiagen) and RNA concentration was measured using a NanoDrop ND-1000 (Labtech International).



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Addgene inc david ron
David Ron, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/david+ron/ATF4+5%3A+5%E2%80%99ATF4%2EGFP+(Plasmid+%2321852)/pm41896223-87-9-11
Average 93 stars, based on 1 article reviews
david ron - by Bioz Stars, 2026-09
93/100 stars
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